An Investigation to Show the Effect of Temperature on Daphnia :: Papers

An investigation to Show the Effect of Temperature on cyclops Plan I pull up stakesing submerge some daphnia contained in a test subway system in water of differing temperatures and measure their subsequent heart rates. Hypothesis I think that the heart rate of the daphnia forget increase up until around 40C at which point most of the daphnias enzymes volition have a bun in the oven denatured and rate of metabolism leave have halt or decreased sufficiently to have stopped the daphnias heart rate. instrument list Test tube Beaker Different temperatures of water Daphnia pipet Stop clock Microscope Slide Safety Follow usual science laboratory safety rules. There are no major safety considerations in this experiment as the daphnia arent harmful and water above 40C will not be used. Method To determine if temperature does have an effect on the daphnia, I intend to carry out the future(a) experiment. The experiment will involve measuring the cardiac activity of the daphnia at different temperatures, ranging from very cold (approx. 5 degrees Celsius) to quite change (approx. 40 degrees Celsius). A selection of similar sized/age Daphnia will be taken. A variety of different temperatures of water will be set up, these temperatures will be kept constant whilst the daphnia are submersed in them. The temperatures will be set up by using ice to cool tap water pile to lower temperatures and boiling water to heat it up. The temperature will be metrical to within 0.1C on a mercury thermometer to ensure accuracy. The temperatures will range from 10C to approximately 40C and it will be done at 5C intervals. The daphnia will be submersed in the water and left to equilibrate for 3 minutes, after this time one of the Daphnia will be outback(a) and endow onto a microscope slide, this will then be quickly put under the microscope. -1- Under the microscope, the Daphnia will be observ ed for 20 seconds, this heart rate will be counted and recorded, this can the be