Analysis of Ethanol in Moonshine

Analysis of Ethanol in Moonshine Using the GC-MS Jennifer Greene - 4 May 2012 - 4 May 2012 Introduction In this experiment spatter chromatography is applied to separate the water-grain alcohol mixture. This method is often used in the finale of alcohol in blood or urine. One obvious practical application is when law enforcement agencies assume to determine whether or not someone is inebriated. In these reasons, high sensitivity is required since 0. 1% blood alcohol heart is considered to be legally intoxicated in most states.Our own determination will deal with higher concentrations (up to 25% by volume) which atomic telephone number 18 much typical of alcohol levels put in in many alcoholic beverages. Chromatography is a physical method of separation in which the components to be separated atomic number 18 distributed between two phases, one of the phases constituting a unmoving bed of tumid surface area, the other being a fluid that percolates through or along the sta tionary bed Gas chromatography (GC) is a powerful and wide used wight for the separation, identification and quantitation of components in a mixture.In this technique, a try out is converted to the vapor state and a flowing stream of pallbearer gun for hire (often helium or nitrogen) sweeps the precedent into a thermally-controlled editorial. In the case of gas-liquid chromatography, the mainstay is usually packed with solid particles that are coated with a non-volatile liquid, referred to as the stationary phase. As the sample mixture moves through the column, sample components that interact strongly with the stationary phase spend more prison term in the stationary phase vs. the moving gas phase and consequently require more time to move through the column.The goal of GC is to separate and detect components of a mixture as efficiently as possible and it is thus desirable to have a measure of the capability of a given chromatographic column. One common way to distil co lumn efficiency is by calculating the height equivalent to a theoretical plate (HETP) which is the space of the column divided by the number of theoretical plates. HETP = l/n The number of theoretical plates (n) of a column with respect to a particular confused can be found using the following equation N = 16(tr/w)2 = 5. 55(tr/w1/2)2Where w is the peak larger-than-lifeness measured in the same units as tr and w1/2 is the peak width measured at half of the peak height. Materials GC-MS with database Vials with lids Cherry moonshine liquid pipettes Ethanol 100% Procedure 1. mass up procedure to Set Detector A On (Thermal Conductivity Detector) Packed Column lead Pressure 30 psi Column Carbowax 20M, 1/8 OD, length = 6 feet Oven Temperature 110C Detector Temperature 150C crack Temperature 150C 2. With the disposable pipette fill separate vials with the cerise moonshine and pure fermentation alcohol. . Run the procedure then define the ethanol peak with three additional peaks. This is the chromatograph for Cherry Moonshine. This is the chromatograph for Cherry Moonshine. Data The glowering arrows are pointing to the ethanol peak. The blue arrows are pointing to the ethanol peak. Conclusion Chromatography is a physical method of separation in which the components to be separated are distributed between two phases, one of the phases constituting a stationary bed of large surface area, the other being a fluid that percolates through or along the stationary bed.Gas chromatography (GC) is a powerful and widely used tool for the separation, identification and quantitation of components in a mixture. In this technique, a sample is converted to the vapor state and a flowing stream of carrier gas (often helium or nitrogen) sweeps the sample into a thermally-controlled column. In the case of gas-liquid chromatography, the column is usually packed with solid particles that are coated with a non-volatile liquid, referred to as the stationary phase.As the sample mixt ure moves through the column, sample components that interact strongly with the stationary phase spend more time in the stationary phase vs. the moving gas phase and thus require more time to move through the column. This experiment demonstrated how the analysis of an extraterrestrial substance can be determined from the separate peaks. If you select an private peak on the chromatograph, the database will bring up possible matches of the compound.You would need to look at the mass spectrogram of that peak to see if it matches the compound of interest. If it does, then you have confirmed one compound that is present in the substance. If it is not a match, continue to search the database until you confirm the peaks identity. From this experiment, the peak for ethanol was not determined using the mass spectrograph of the gas chromatography however, we ran pure ethanol on the GC-MS and compared the two chromatographs to determine the ethanol peak.